Review



h2b sequence to pag1256  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Addgene inc h2b sequence to pag1256
    a Constructs for the expression of bJun-pFAST 1-98 , bFos-pFAST 115-125 and <t>H2B-pFAST</t> 99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST 1-98 , <t>H2B-pFAST</t> 99-114 and either bFos-pFAST 115-125 or bFosΔ(179-193)-pFAST 115-125 . Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST I F (tripartite-split-FAST) with the fluorescence intensity I F (iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.
    H2b Sequence To Pag1256, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h2b+sequence+to+pag1256/pAAV-TRE-mRuby2+(Plasmid+%2399114)/pmc12307597-277-17-36
    Average 92 stars, based on 5 article reviews
    h2b sequence to pag1256 - by Bioz Stars, 2026-11
    92/100 stars

    Images

    1) Product Images from "Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes"

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

    Journal: Nature Communications

    doi: 10.1038/s41467-025-62241-8

    a Constructs for the expression of bJun-pFAST 1-98 , bFos-pFAST 115-125 and H2B-pFAST 99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST 1-98 , H2B-pFAST 99-114 and either bFos-pFAST 115-125 or bFosΔ(179-193)-pFAST 115-125 . Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST I F (tripartite-split-FAST) with the fluorescence intensity I F (iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Constructs for the expression of bJun-pFAST 1-98 , bFos-pFAST 115-125 and H2B-pFAST 99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST 1-98 , H2B-pFAST 99-114 and either bFos-pFAST 115-125 or bFosΔ(179-193)-pFAST 115-125 . Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST I F (tripartite-split-FAST) with the fluorescence intensity I F (iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.

    Techniques Used: Construct, Expressing, Imaging, Comparison, Fluorescence

    Related Articles

    Plasmid Preparation:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.

    Expressing:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.

    Construct:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.

    Sequencing:

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
    Article Snippet: The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB . .. The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 . .. The plasmid pAG1258 allowing the mammalian expression of myc-bFos-pFAST 115-125 -IRES-HA-iRFP670 was constructed by replacing FKBP sequence from pAG1151 by bFos sequence from Addgene vector #22013 pBiFC-bFosVC155.



    Similar Products

    h2b sequence to pag1256  (Addgene inc)
    92
    Addgene inc h2b sequence to pag1256
    a Constructs for the expression of bJun-pFAST 1-98 , bFos-pFAST 115-125 and <t>H2B-pFAST</t> 99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST 1-98 , <t>H2B-pFAST</t> 99-114 and either bFos-pFAST 115-125 or bFosΔ(179-193)-pFAST 115-125 . Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST I F (tripartite-split-FAST) with the fluorescence intensity I F (iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.
    H2b Sequence To Pag1256, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h2b+sequence+to+pag1256/pAAV-TRE-mRuby2+(Plasmid+%2399114)/pmc12307597-277-17-36
    Average 92 stars, based on 1 article reviews
    h2b sequence to pag1256 - by Bioz Stars, 2026-11
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    a Constructs for the expression of bJun-pFAST 1-98 , bFos-pFAST 115-125 and H2B-pFAST 99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST 1-98 , H2B-pFAST 99-114 and either bFos-pFAST 115-125 or bFosΔ(179-193)-pFAST 115-125 . Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST I F (tripartite-split-FAST) with the fluorescence intensity I F (iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

    doi: 10.1038/s41467-025-62241-8

    Figure Lengend Snippet: a Constructs for the expression of bJun-pFAST 1-98 , bFos-pFAST 115-125 and H2B-pFAST 99-114 for probing the Fos·Jun·DNA ternary complex formation. b Representative confocal micrographs of HBR-2,5DM-treated HeLa cells co-expressing bJun-pFAST 1-98 , H2B-pFAST 99-114 and either bFos-pFAST 115-125 or bFosΔ(179-193)-pFAST 115-125 . Cells were treated with 10 μM HBR-2,5DM. Identical imaging settings were used for direct comparison. Scale bars 20 μm. Representative confocal micrographs of n > 100 cells from three independent experiments. c Relative nuclear tripartite-split-FAST fluorescence computed by normalizing the nuclear fluorescence intensity of tripartite-split-FAST I F (tripartite-split-FAST) with the fluorescence intensity I F (iRFP670) of co-expressed iRFP670. Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the three biological replicates. Source data are provided as a Source Data file.

    Article Snippet: The plasmid pAG1713 allowing the mammalian expression of H2B-pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-HA-mTurquoise2 was constructed by adding H2B sequence to pAG1256 (CMV pFAST 99-114 -P2A-bJun-pFAST 1-98 -IRES-mTurquoise2, which was itself constructed by adding bJun sequence from Addgene vector #22012 pBiFC-bJunVN173 to pAG1184) for expression of a fusion between H2B and pFAST 99–114 .

    Techniques: Construct, Expressing, Imaging, Comparison, Fluorescence